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Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM
Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
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Development of DNA probe for detection of Aujeszky's disease virus Arq. Bras. Med. Vet. Zootec.
Cândido,A.L.; Barros,P.P.; Resende,M.; Flaschart,R.B..
A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky's disease virus; Dot-blot hybridization; Radioactive probe; Non radioactive probe.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001
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Differential detection of transposable elements between Saccharum species Genet. Mol. Biol.
Souza,Marislane Carvalho Paz de; Silva,Jéssica Naiana; Almeida,Cícero.
Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analyze the repetition of DNA sequences in S. officinarum and S. spontaneum. For this purpose, genomic DNA from S. officinarum was digested with restriction enzymes and the fragments cloned. Sixty-eight fragments, approximately 500 bp, were cloned, sequenced and had their identity analyzed in NCBI, and in the rice, maize, and sorghum genome databases using BLAST. Twelve clones containing partial transposable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugarcane; Dot-blot hybridization; LTR retrotransposons; Repetitive DNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300016
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